54 Agilent 1200 Preparative Pump User Manual
4 Using the Preparative Pump
Prevent Blocking of Solvent Filters
Prevent Blocking of Solvent Filters
Contaminated solvents or algae growth in the solvent bottle will reduce the
lifetime of the solvent filter and will influence the performance of the module.
This is especially true for aqueous solvents or phosphate buffers (pH 4 to 7).
The following suggestions will prolong lifetime of the solvent filter and will
maintain the performance of the module.
• Use a sterile, if possible amber, solvent bottle to slow down algae growth.
• Filter solvents through filters or membranes that remove algae.
• Exchange solvents every two days or refilter.
• If the application permits add 0.0001-0.001M sodium azide to the solvent.
• Place a layer of argon on top of your solvent.
• Avoid exposure of the solvent bottle to direct sunlight.
• Filter HPLC grade (dry) Acetonitrile before use. The dryer the Acetornitrile,
the stronger the tendency to form polymers and therefore block the system.
Refilter at least every two days.
Never use the system without solvent filter installed.
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