66 Agilent 1200 Preparative Pump User Manual
5 Optimizing Performance
How to Configure the Seal Wash Function
How to Configure the Seal Wash Function
Always use the seal wash function which is delivered with your pump.
Water or Aqueous Buffer Solutions
We recommend a composition of 10% Isopropanol in HPLC grade water for
water or aqueous buffer solutions. With buffer solutions, the seal wash pump
should be configured such, that it is turned on for a duration of 30 s every
5 minutes (with a maximum of 30% cycle time). Shorten the intervals between
the pump turn-ons for highly concentrated buffer solutions (> 0.1 M buffer
solutions).
Organic Solvents
For all organic non buffered solutions, we recommend to use the same solvent
for the seal wash as the one that is currently pumped by your preparative
pump. In this case, the seal wash pump has to be operated in large intervals,
only. Basically it is enough to keep the back of seals wet with the help of the
seal wash option.
The seal wash function cannot be operated permanently (danger of overheating). Switch on
the seal wash pump in regular intervals (normally for 30 s every 30 minutes) for a certain
length of time. It is however important to keep the back of the seals wet with the help of the
seal wash function.
Beware that the seal wash tubing is made of silicon. Never use any solvents for the seal
wash, that are not compatible with silicone.
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