Purification Solution - Developer's Guide 31
Setting Up an Analytical and Preparative System
3
Setting up a preparative system
• In the Detectors section, select the relevant detectors from the available
detectors in the instrument configuration. The system schematics
graphic corresponds to the selected detectors.
4 In the Sampler tab
• The tab is present only for flow-through samplers such as the G7157A
1260 Infinity II Prep Autosampler. There, the volumes of the sample loop
and seat capillary contribute to the time that the sample needs to reach
the column. The volumes must be imported from the current preparative
instrument configuration. Previously created preparative system settings
may refer to a different sample loop or seat capillary and then are
displayed with a warning sign.
5 In the Delay Volumes tab:
• Select the tubing by selecting a system type.
If you select a custom system type, you need to determine the tubing
volumes. For details about determining delay volumes and times, see
Characterizing the delay volumes and Fraction collector delay time and
volume calibration (“Characterizing the delay volumes: Mixing point to
UV detector and Column to UV detector” on page 48, “System
Configuration and Delay Time Calibration” on page 70).
• Specify the additional detectors that are used. If an MSD is part of the
system, specify the delay time between the UV detector and the MSD, and
click Do calibration run to determine the delay time between the UV
detector, fraction collector and MSD.
6 Switch to the Column tab and provide details of the preparative column.
7 Click Save System to save the preparative system parameters.
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