Purification Solution - Developer's Guide 75
Calibration Procedures
6
System Configuration and Delay Time Calibration
Configuring the Preparative System Parameters
In the Purification menu of the Chemstation, select Systems to open the System
Parameters dialog box. The preparative system parameters are on the right of
the divided window. Click Add system to add a new system, and set the
following:
1 List of systems:
• Name and description of the system
2 Pumps and Detectors tab:
• In the Pumps section:
• Select the Main Pump.
• Select the preparative pump module (cluster) and specify the solvent
channels.
• Set the preparative flow.
• If a make-up splitter is used, mark Use make-up pump and select the
pump module. Select the make-up solvent Channel and set the make-up
Flow.
• In the Detectors section:
• Mark the Use MSD check box if the MSD is used to trigger fraction
collection.
• Mark the Use auxiliary detector check box if an auxiliary detector is
used, and select the module.
• Select the UV detector module that will be used for evaluation of the
preparative UV signal. This detector can be located in the preparative
flow path or in the make-up flow path, depending on the instrument
configuration.
• Select the Peak width mode of the UV detector. The recommended
frequency value is 2.5 Hz if the Agilent active make-up splitter or
slope-based fraction triggering are used. Otherwise, 20 Hz is
recommended.
• Mark the UV detector located behind splitter check box if the UV detector
is located in the make-up flow path. This check box can be set only if
Custom Tubing is selected in the Delay Volumes tab.
3Delay Volumes tab:
• Tubing
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