76 Purification Solution - Developer's Guide
6
Calibration Procedures
System Configuration and Delay Time Calibration
• Select the instrument tubing setup from the drop-down list:
• Selecting a predefined system fills tubing volumes and the position
of the UV detector in relation to the make-up splitter if an MSD is
used.
• If you are using a custom system, measure and/or calculate the
tubing volumes as described in Characterizing the tubing volumes
(“Characterizing the delay volumes: Mixing point to UV detector
and Column to UV detector” on page 48).
• Delays and fraction collector calibration
• Choose the automated option in Fraction collector calibration to perform a
calibration run as described in “Delay time calibration for Preparative
Systems” on page 77.
• Delay fields are editable only if the manual option is selected. Also, if
an MSD or auxiliary detectors are not set up, the related fields are not
editable.
• To enable the entry of known delays in Preparative systems, Fraction
collector calibration must be set to manual.
• If the manual option is selected and Calculate delays to fraction collector is
marked, then Time MSD to fraction collector and Time auxiliary detector to
fraction collector are calculated from other delays (UV to FC and UV to
detector). If Calculate delays to fraction collector is cleared, then they can
also be edited, and you must ensure integrity of entered delays (due to
interconnection of UV to FC, UV to detector and detector to FC).
• Write delay times stores the entered detector-to-fraction collector delay
times to the instrument.
4Column tab:
• Enter the name and parameters of the column.
• Void volume and porosity are recalculated to each other when edited
using the diameter of the column (porosity is the ratio of void and
geometrical column volume).
The Write delay times action is not mandatory but then the delay time values have to be
written to the instrument at the beginning of every task and the original values are
restored after the task is finished. This can significantly prolong the overall run time.
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