74 Purification Solution - Developer's Guide
6
Calibration Procedures
System Configuration and Delay Time Calibration
• Initial Pressure: the 1260 quaternary analytical pump has a dwell
volume dependent on the actual pressure. In order to minimize any
possible discrepancy, enter the initial pressure on the pump at the
gradient start conditions.
• Delays
• If the delays for Analytical systems are required for MSD and/or
auxiliary detectors, follow the instructions in “Delay time
determination for Analytical systems (MSD and/or auxiliary
detectors)” on page 74
4Column tab:
• Enter the name and parameters of the column.
• Void volume and porosity are recalculated to each other when edited
using the diameter of the column (porosity is the ratio of void and
geometrical column volume).
Delay time determination for Analytical systems (MSD and/or
auxiliary detectors)
1 Acquire an analytical run in Chemstation as described in “Acquire an
analytical run using the Chemstation sequence” on page 85.
2 Go to Data Analysis in Chemstation and load the acquired data.
3 Calculate the delays, for example, using the caffeine peak (the first major
analyte peak) based on the elution times in all detectors used:
• Based on the extracted EIC 195 ion trace from MSD1 TIC signal
calculate: Time UV detector to MSD = MSD1 EIC 195 signal – UV detector
signal
• Time UV detector to auxiliary detector = UIB1 A (Analog In) – UV
Detector signal
4 Enter the values into the respective fields in the Analytical systems dialog
box.
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