Purification Solution - Developer's Guide 85
Checkout Procedure
7
Acquire an analytical run using the Chemstation sequence
Acquire an analytical run using the Chemstation sequence
1 Set up a generic gradient in the analytical method:
a If using a combined analytical and preparative system:
a Use the Analytical_Purification method setting as described in
“Preparing Default Purification Methods” on page 9.
b Save the method as
Analytical_Gradient.
b Enter the recommended generic gradient in the method:
• Pump > Solvents > B: 2 %
• Pump > Timetable:
For an Agilent 1260 Infinity analytical run using a 4.6 × 50 mm, 5 μm
column and a Flow of 1 mL/min:
For an Agilent 1290 Infinity analytical run with a binary pump using a
2.1 × 50 mm, 1.8 μm column and a Flow of 0.6 mL/min:
If the analytical run is to be acquired as a single run for the Purification software, it is
important to mark the Save Method with Data check-box in Method > Runtime Check
List. This applies to both the combined preparative instrument and the stand-alone
analytical instrument.
Time (min) A (%) B (%)
0.00 98 2
0.50 98 2
5.30 2 98
6.40 2 98
6.50 98 2
Stoptime: 9.0 min
To Next Page
Loading...
Need help?
General
