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Purification Solution - Developer's Guide
Agilent Technologies
6
Calibration Procedures
Characterizing the delay volumes: Mixing point to UV detector and
Column to UV detector 48
Tubing Volumes for Predefined Systems 48
Calculation of delay volume: Column to UV detector 50
Calculation of delay volume: Mixing point to UV detector 52
Experimental measurement of delay volume: Mixing point to UV
detector 57
Determining the elution time of acetone 60
Characterizing the column volume 63
Column Volume: Standard Analytical System 63
Combined Analytical and Preparative System 66
System Configuration and Delay Time Calibration 70
Running conditions for calibration procedures 71
Configuring the Analytical and Preparative Systems 73
Delay time determination for Analytical systems (MSD and/or
auxiliary detectors) 74
Configuring the Preparative System Parameters 75
Delay time calibration for Preparative Systems 77
Insufficient UV-to-FC delay time or MSD-to-FC delay time 80
This chapter gives step-by-step instructions for the important calibration
procedures for the Purification system.
Before starting the calibration procedure(s), check that all prerequisites are
available (solvents, samples and others). The list is placed in the beginning of
each document.
The calibration procedures are a set of measurements and calculations that
calibrate all required parameters for the Purification software. The work-flow
is as follows:
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