52 Purification Solution - Developer's Guide
6
Calibration Procedures
Characterizing the delay volumes: Mixing point to UV detector and Column to UV detector
Calculation of delay volume: Mixing point to UV detector
The tables provided in these sections describe the predefined capillaries, UV
cells and sampler loop configurations, and can be used to calculate custom
setups. An approximate value of the Mixing point to UV detector tubing
volume can be calculated if the volumes of all modules and capillaries are
known:
Standard Analytical System
Due to the damper used in the 1100 and 1200 series pumps (G1311A/B and
G1312A/B), the pump's dead volume is dependent on pressure, solvent type
and composition. The following table summarizes the volumes of some 1100,
1200, 1260 and 1290 pumps and autosamplers with their optional set-ups. All
listed autosamplers except the dual loop autosampler (G2258A) have their
metering devices in the main flow path; therefore, their volumes vary with the
sample injection volume.
The calculation of the Mixing point to UV detector tubing volume consists of
the following volumes:
• Pump (including mixer, if installed)
• Autosampler (Injector)
• Capillaries
• Pump to autosampler
• Autosampler to column
Tabl e 7 Volume in µL per mm of some stainless steel and PEEK capillaries
Stainless Steel
PEEK
ID [mm] µL/mm ID [mm] µL/mm
0.12 0.0113 0.1 (0.004") 0.0081
0.17 0.0227 0.13 (0.005") 0.0127
0.25 0.0491 0.18 (0.007") 0.0248
0.5 0.196 0.25 (0.01") 0.0507
0.6 0.283 0.51 (0.02") 0.203
0.7 0.385 0.76 (0.03") 0.456
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