50 Purification Solution - Developer's Guide
6
Calibration Procedures
Characterizing the delay volumes: Mixing point to UV detector and Column to UV detector
“Calculation of delay volume: Mixing point to UV detector” on page 52. The
capillaries are delivered in the respective capillary kit. If you have a custom
tubing setup, use the tables as templates.
Calculation of delay volume: Column to UV detector
The tables provided below describe the predefined capillaries, UV cells and
sampler loop configurations, and can be used to calculate custom setups. The
Volume column to UV detector tubing volume consists of all tubing between
the column and UV detector cell (including the UV cell inlet tubing).
For a standard analytical system the Volume column to UV detector typically
consists of the following volumes:
• Column to UV detector capillary
• Inlet capillary of the UV detector cell
• Dead volume of the UV detector cell
Calculation of the Column to UV detector delay volume of predefined
purification systems:
Tabl e 4 Volume in µL per mm of some stainless steel and PEEK capillaries
Stainless Steel
PEEK
ID [mm] µL/mm ID [mm] µL/mm
0.12 0.0113 0.1 (0.004") 0.0081
0.17 0.0227 0.13 (0.005") 0.0127
0.25 0.0491 0.18 (0.007") 0.0248
0.5 0.196 0.25 (0.01") 0.0507
0.6 0.283 0.51 (0.02") 0.203
0.7 0.385 0.76 (0.03") 0.456
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