Purification Solution - Developer's Guide 73
Calibration Procedures
6
System Configuration and Delay Time Calibration
Example of F
prep
calculation for two columns of the same porosity:
• Analytical column 4.6 x 50 mm (d
anal
= 4.6 mm) with d
p,anal
= 5 μm particle
diameter
• Preparative column 21.2 x 50 mm (d
prep
= 21.2 mm) with d
p,prep
= 5 μm
particle diameter
• Analytical flow F
anal
= 1 mL/min
The resulting scaled-up preparative flow F
prep
= 1 × 21.2
2
/ 4.6
2
× 5 / 5 = 21
mL/min.
Configuring the Analytical and Preparative Systems
In the Purification menu of the Chemstation, select Systems to open the System
Parameters dialog box. The analytical system parameters are on the left of the
divided window. Click Add system to add a new system, and set the following:
1 List of systems:
• Name and description of the system
2 Pumps and Detectors tab:
• Select the System Type
• In the Pumps section:
• Select the Main Pump
• Select the analytical pump module and specify the solvent channels.
• Set the analytical flow.
3Delay Volumes tab:
• Tubing
• Select the instrument setup from the drop-down list:
• Selecting a predefined system fills tubing volumes and the position
of the UV detector in relation to the splitter if an MSD is used.
• If you are using a custom system, measure and/or calculate the
tubing volumes as described in “Characterizing the delay volumes:
Mixing point to UV detector and Column to UV detector” on
page 48.
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