83
Purification Solution - Developer's Guide
Agilent Technologies
7
Checkout Procedure
Acquire an analytical run using the Chemstation sequence 85
UV-only Workflow: Focused gradient in the Purification software 89
UV/MS Workflow: Focused gradient in Purification software 92
This chapter gives step-by-step instructions on how to carry out a checkout
procedure to confirm the correct operation of your Purification system.
Chemicals For all systems (stand-alone analytical, preparative or combined analytical
and preparative):
• Solvent A: water with 0.1 % formic acid
• Solvent B: acetonitrile with 0.1 % formic acid
• Make-up solvent (used with splitter): 80 % acetonitrile in water with 0.1 %
formic acid
• Needle purge and wash solution (degas in ultrasonic bath): 80 % acetonitrile
or other suitable solution
• DMSO (dimethyl sulfoxide) for sample dilution
Columns • Analytical (recommended): 4.6 × 50 mm, 5 μm (846975-902)
• Preparative (recommended): 21.2 × 50 mm, 5 μm (870050-902 with end
fittings 820400-901)
Sample • Prep LC Standards #1 (5190-6886) containing check-out mixture. For the
analytical run, dilute the sample with DMSO (or other compatible solvent)
based on the UV cell length:
• 3mm UV cell (10μL injection with dual-loop autosampler): Dilute 10×
(1 mL = 100 μL sample + 900 μL solvent)
• 10 mm UV cell (10 μL injection with dual-loop autosampler): Dilute 40×
(1 mL = 25 μL sample + 975 μL solvent)
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