92 Purification Solution - Developer's Guide
7
Checkout Procedure
UV/MS Workflow: Focused gradient in Purification software
UV/MS Workflow: Focused gradient in Purification software
This procedure is the analytical-to-preparative workflow for the combined
analytical and preparative UV/MSD instrument and for standalone
preparative UV/MSD instrument.
1 Place at least 1.5 mL of filtered undiluted Prep LC Standards #1 sample in
the autosampler.
2 For the dual loop autosampler:
a Switch the valve to the upper loop (right-click on the autosampler
diagram and select Switch Valve to Upper Loop; if the command is not
available, the upper loop is already selected).
b If the autosampler has been idle for more than one hour:
• Wash the needle (right-click on the autosampler diagram and select
Wash Needle > Flush Port > 15 s)
• Purge the needle (right-click on the autosampler diagram and select
Start Purging > 3×)
3 Precondition the preparative column if it has not been used recently:
a Set the preparative flow and set 95 % of solvent B (default flow:
20 mL/min for 21.2 × 50 mm column and 1 mL/min analytical run flow).
b Turn on all pumps.
c Apply 95 % of solvent B for approx. 1 min.
d Apply 2 % of solvent B until UV signal and pressure are stable.
e Turn off all pumps.
4 In the Chemstation, open the Tas ks dialog box from the Purification menu.
5 Create a new task:
• Click Add Task.
• Select Add empty task.
• Select Analytical to preparative workflow.
• Click OK
6 Select the Task Configuration tab.
• Select System
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