Purification Solution - Developer's Guide 93
Checkout Procedure
7
UV/MS Workflow: Focused gradient in Purification software
• Select the analytical and preparative systems from “Configuring the
Analytical and Preparative Systems” on page 73.
• Review the analytical and preparative flows in the Pumps and Detectors
tab if the target flows are set.
• Ion Species
• Analytical run: mark +H and –H species for the analytical run.
• Preparative run: mark Use best performing ion from analytical run.
• Analytical Run
• Click Browse and select the acquired analytical sequence
(Checkout_Sample)
• Scale up
• In Gradient, keep the Initial concentration: 2 % check box marked.
• Preparative Run
• Select the Preparative base method (Prep_Purification)
• Keep Review analytical results before purification marked.
• Set the preparative vial Location.
• Set the Inj. volume to 500 μL.
• Fraction collection tab (in Preparative Run)
• Select Tri gger mo de > Threshold for both MSD and UV detectors.
7 Click Run.
8 Set the parameters in Analytical Results:
• Enter a Ta rge t ma ss of 166.1 (Ethyl 4-hydroxybenzoate).
• Click Re-evaluation to apply the change.
• Set suitable thresholds in the Fraction collection tab:
• MSD detector (if available):
• Display MSD ion species curve in the graph.
• Zoom to baseline and determine its highest level.
• Set the
Threshold value to five times the determined analytical
baseline level or 2000, whichever is higher.
• UV detector: set Threshold to 5 or adjust the value to achieve a
threshold of about 5 % of the peak height in the preparative run.
9 Review the Analytical Results (check the settings and the target peak):
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