24 Purification Solution - Developer's Guide
2
Preparing Default Purification Methods
Critical Method Parameters
Critical Method Parameters
In some situations, incorrect settings of the following parameters can cause
incorrect functioning of the Purification software, incorrect fraction
collection, or damage or contamination to some parts of the system. Therefore,
if the purification system does not work correctly, ensure that the following
parameters are set correctly:
Configuration • Fraction collector: Linked pump > select preparative pump
General default
method
• Pump: Pressure limits (to avoid column damage)
• Autosampler: Needle wash (to avoid autosampler seat contamination)
• Autosampler: Vial/Well Bottom Sensing (use only with suitable sample wells
or vials)
• Valve: Position > Use valve position (necessary for Purification software)
• Valve: Position switch at end of run > Do not switch (to avoid system
overpressure due to flow path switch on the 2/10 valve)
• UV Detector: Signals > Set Signal A (set a wavelength suitable for analyzed
compounds)
• MSD: Set Up MSD Signals > General > mark Active Signals (to collect MSD data)
• MSD: MSD Signal Settings > Mode > Scan (necessary for Purification
software)
Analytical default
method
(combined
system)
• Valve 2/10 > Position > Use valve position > select correct analytical position
• Iso. Pump: Flow > 0 mL/min (make-up not used in analytical; flow can cause
leak sensor error)
• UIB II: Expected ERI Mode > No mode check (for use with MSD, to avoid not
ready state in some cases)
• Autosampler Plug: Plug solvent should contain only minimum amount of
organic solvent to avoid unwanted partial elution of the sample on the
column causing peak distortion and/or elution time variability
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