Purification Solution - Developer's Guide 19
Preparing Default Purification Methods
2
Preparative Method Settings
Preparative Method Settings
Load the general settings of the purification method:
General_Purification(“General Method Settings” on page 11)
Save the method as
Prep_Purification:
• Method > Save Method As.
Isocratic Pump (G1310B)
Separated preparative MSD system — make-up pump
Quaternary Pump (G1311B)
Combined analytical and preparative MSD system — as make-up pump:
Prep Pump and Prep Pump Cluster (G7161A/B, G1361A and G1391A)
Flow 1.5 mL/min
Advanced Set Maximum Flow Gradient to about 10 times the target flow.
Channel D 100 %
Flow 1.5 mL/min
Advanced Set Maximum Flow Gradient to about 10 times the target flow.
Flow 20 mL/min (scale-up of 4.6 × 50 mm to 21.2 × 50 mm columns
with 5 µm particle size).
Advanced Channel A and B In Maximum Flow Gradient, set both Flow ramp up and Flow
ramp down to about 10 times the target preparative flow.
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