20 Purification Solution - Developer's Guide
2
Preparing Default Purification Methods
Preparative Method Settings
Dual Loop Autosampler (G2258A)
Prep Sampler (G7157A)
Valve 2/10 (G1170A and G4730A)
UV (DAD, MWD, VWD)
• For instruments with Agilent active splitter or if using the slope-based
collection mode, set Peakwidth to:
• for DAD and MWD: > 0.1 min (2 s response time) (2.5 Hz)
• for VWD: > 0.2 min (4 s response time) (2.5 Hz)
UIB II (G1390B)
If an MSD is configured:
• G1364B: Expected ERI Mode: MSD
• G7159B: No mode check
If the Agilent Active Splitter (G1968D) is connected to the external contacts of
the UIB II:
• Timetable:
• 0, Change contacts, Contact A state: Closed
• 0.01, Change contacts, Contact A state: Open.
• External Contacts: Contact A: mark Closed
Injection Loop Upper
Injection mode Partial Loop Filling
Injector Cleaning Rinse volume: 1 (increase to 5 for injections below 200 µL)
Advanced Draw speed and Eject speed: 8000.0 µL/min (decrease to 3000.0
for injections below 200 µL)
Advanced Set Draw Speed to 3000.00 µL/min.
Position Use valve position: set the preparative position
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