70 Purification Solution - Developer's Guide
6
Calibration Procedures
System Configuration and Delay Time Calibration
System Configuration and Delay Time Calibration
Chemicals • Solvent A: water with 0.1 % formic acid
• Solvent B: acetonitrile with 0.1 % formic acid
• Make-up solvent: 80 % acetonitrile in water with 0.1 % formic acid
• Needle purge and wash solution (degas in ultrasonic bath): 80 % acetonitrile
or another suitable solution
Sample For G1364B
• Prep LC Standards #2 (5190-6887) containing blue dye thionine acetate.
Dilute the sample with pure water based on purification instrument setup,
and it is recommended that you filter the sample with the regenerated
cellulose syringe filter (5190-5108).
• Preparative UV or UV/MSD instrument with 3 mm UV cell:
Dilute 20 times (1 mL = 50 μL sample + 950 μL water).
• Preparative UV or UV/MSD instrument with 0.3 or 0.06 mm UV cell:
Dilute 10 times (1 mL = 100 μL sample + 900 μL water).
• Combined preparative instrument:
Dilute five times (1 mL = 200 μL sample + 800 μL water).
For G7159B, G1364E and G7166A
• Agilent Delay and Checkout Calibrant (5190-8223) containing a mixture
with Patent Blue VF dye. It is recommended that you filter the sample with
the PTFE syringe filter (5190-5084).
The delay time calibration determines a delay of a signal between a fraction
collector (FC) and a detector.
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