82 Purification Solution - Developer's Guide
6
Calibration Procedures
System Configuration and Delay Time Calibration
also, a longer coil with the same inner diameter will increase
back-pressure).
• Decrease the preparative flow (influences the chromatographic resolution).
• Increase the make-up flow (increases speed of the analytes when passing
through detectors, which typically results in narrower and/or smaller
peaks, so consider whether or not the detector data rate is sufficient for the
signal response).
• Decrease the volume of tubing between the splitter and the detector(s).
After one or more solutions have been applied, repeat the fraction collector
delay calibration from the beginning.
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