84 Purification Solution - Developer's Guide
7
Checkout Procedure
System Configuration and Delay Time Calibration
• 10 mm UV cell (1 μL injection): Dilute 20× (1 mL = 50 μL sample + 950 μL
solvent)
• 60 mm UV cell (1 μL injection): Dilute 100× (1 mL = 10 μL sample +
990 μL solvent)
• Plug vial solvent: 2 % acetonitrile (or Solvent A or other compatible
solvent). Refresh the solvent frequently to avoid bacterial growth and
injection cross-contamination.
• Recommended: For best column performance and lifetime, filter the sample
in DMSO solvent before use with the PTFE syringe filter (5190-5084). Filter
other sample solvents (water, acetonitrile) such as the plug solvent with the
regenerated cellulose syringe filter (5190-5108). To select the most suitable
filter type with the highest sample recovery, filter the sample solvent matrix
without the sample first.
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