Purification Solution - Developer's Guide 49
Calibration Procedures
6
Characterizing the delay volumes: Mixing point to UV detector and Column to UV detector
For the combined preparative and analytical MSD default setup, the tubing
volumes of the analytical flow path are dependent on pressure, solvent type
and composition. This is due to the presence of the dampener in the
quaternary pump G1311A/B/C used with this system. Therefore, by selecting
one of the predefined combined systems, an Initial pressure field is provided in
the in the Delay Volumes tab of the Analytical Systems dialog box. The Volume
mixing point to UV detector tubing volume is updated based on the value of Initial
pressure.
Default capillaries, UV cells and sample loops are listed in the tables in
“Calculation of delay volume: Column to UV detector” on page 50 and
Tabl e 1 List of predefined combined setups in Analytical Systems dialog box
Setup Description
Combined UV/MSD,
G1311B, ACN
Analytical flow path of the default combined UV/MSD
analytical-to-preparative instrument setup with G1311B quaternary
pump and 50 µL loop in the dual-loop autosampler (G2258A).
Acetonitrile or methanol as solvent for the pump channel B.
Combined UV/MSD,
G1311B, MeOH
Combined UV/MSD,
G1311A/C, ACN
Analytical flow path of the default combined UV/MSD
analytical-to-preparative instrument setup with G1311A or G1311C
quaternary pump and 50 µL loop in the dual-loop autosampler
(G2258A). Acetonitrile or methanol as solvent for the pump
channel B.
Combined UV/MSD,
G1311A/C, MeOH
Tabl e 2 List of predefined MSD-based setups in Preparative Systems dialog box
Setup Description
Combined UV/MSD Preparative flow path for the default combined UV/MSD
analytical-to-preparative instrument setup with 3 mm preparative
UV cell and 5 mL loop in the dual-loop autosampler (G2258A).
Preparative UV/MSD
default setup; 3 mm UV cell
Preparative UV/MSD instrument setup with 3 mm preparative UV
cell and 5 mL loop in the dual-loop autosampler (G2258A).
Tabl e 3 List of predefined UV-only setups in Preparative Systems dialog box
Setup Description
Preparative UV-only default
setup; 3 mm UV cell
Preparative UV instrument setup with 3 mm preparative UV cell
and 5 mL loop in the dual-loop autosampler (G2258A).
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