Purification Solution - Developer's Guide 51
Calibration Procedures
6
Characterizing the delay volumes: Mixing point to UV detector and Column to UV detector
Tabl e 5 UV/MSD-based combined analytical and preparative 1260 LC system
Analytical Volume, mL Preparative with bypassed
splitter
Volume, mL
Column to valve (0.17 × 400
mm)
0.009 Column to splitter (0.5 × 600
mm)
0.118
Valve to UV (0.17 × 400 mm) 0.009 Splitter bleed bypass (2 × 0.25
× 80 mm)
0.008
UV inlet tubing for 3 mm Prep
Flow Cell (0.5 × 250 mm)
0.050 Splitter to valve (0.18 × 1400
mm)
0.034
Valve to UV (0.17 × 400 mm) 0.009
UV inlet tubing for 3 mm Prep
Flow Cell (0.5 × 250 mm)
0.050
Total 0.068 Total 0.219
Tabl e 6 UV/MSD-based system with UV detector in front of splitter or UV-based
preparative 1260 LC system
Preparative with bypassed splitter Volume, mL
Column to UV (0.5 × 600 mm) 0.118
UV inlet tubing for 3 mm Prep Flow
Cell
0.050
1
1
Note that the inlet tubing of 0.06 mm (or 0.3 mm) preparative UV cell does not have a fixed length
(the tubing 0.5x800 or 0.8x2000 mm provided should be cut before use). UV inlet tubing cell vol-
ume has to be adjusted in the table.
To tal 0 .16 8
The inlet tubing of the 0.06 mm (or the 0.3 mm) Preparative UV flow cell does not have a
fixed length. We suggest that you cut the 0.5 × 800 mm and 0.8 × 2000 mm tubing
provided before use.
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