Agilent Technologies 1260 Infinity Quick Start Guide

Purification Solution - Developer's Guide 53
Calibration Procedures
6
Characterizing the delay volumes: Mixing point to UV detector and Column to UV detector
• If used, heat exchange capillary in the thermostat of the column
compartment (TCC)
• Column to detector
• Inlet capillary of the UV detector cell
• Dead volume of the UV detector cell
Dead volumes of
Agilent pumps
The dead volume of some modules is dependent on pressure and solvent;
therefore, the table contains a calculation of the dead volumes for
Water/Acetonitrile and Water/Methanol gradients using the initial pump
pressure (p
0
, bar) at the beginning of the gradient.
Dead volumes of
Agilent
autosamplers
Because of the flow-through design, the injected volume must be added to the
module’s dead volume.
Pump Volume, mL
Water/ACN (p
0
, bar)
Volume, mL
Water/MeOH (p
0
, bar)
G1311A/C
G7111A
1120 Compact LC
1220 VL Compact LC
p
0
/2000 + 0.76 p
0
/4000 + 0.82
G1311B
G7111B
1220 Compact LC
p
0
/2760 + 0.742 p
0
/6060 + 0.787
G5611A p
0
/2860 + 0.725 p
0
/2860 + 0.725
G1312A/C p
0
/3330 + 0.76 0.865
G1312B
G7112B
No mixer: p
0
/20000 + 0.205
Mixer: p
0
/4000 + 0.77
No mixer: 0.225
Mixer: p
0
/6670 + 0.83
G4204A
G7104A/C
No mixer: 0.46
V380: 0.85
No mixer: 0.46
V380: 0.85
G4220A/B
G7120A
No mixer: 0.033
V35: 0.075
V100: 0.16
V380: 0.39
No mixer: 0.033
V35: 0.075
V100: 0.16
V380: 0.39

Agilent Technologies 1260 Infinity Specifications

General

Brand	Agilent Technologies
Model	1260 Infinity
Category	Laboratory Equipment
Language	English