54 Purification Solution - Developer's Guide
6
Calibration Procedures
Characterizing the delay volumes: Mixing point to UV detector and Column to UV detector
Example of the
calculation of the
Mixing point to
UV detector delay
tubing volume
Calculation for a custom 1290 Infinity binary system in a set-up using a
thermostat capillary in the TCC module:
Autosampler Syringe: Volume, mL
G7129A/B/C
G7167A/B
40 µL: 0.04 + injected volume
100 µL: 0.062 + injected volume
900 µL: 0.177 + injected volume
G4226A 20 µL: 0.08 + injected volume
40 µL: 0.118 + injected volume
100 µL: 0.3 + injected volume
G1377A 8 µL: 0.048 + injected volume
40 µL: 0.142 + injected volume
G1367E 40 µL: 0.118 + injected volume
100 µL: 0.3 + injected volume
G1367D 40 µL: 0.142 + injected volume
100 µL: 0.3 + injected volume
G1313A
G1329A/B
G1367A/B/C
G5667A
1120 Compact LC
1220 VL Compact LC
1220 Compact LC
100 µL: 0.3 + injected volume
G5668A 100 µL: 0.062 + injected volume
Part Description Volume, mL
Pump 1290 Infinity Binary Pump with V100 mixer 0.160
Capillary Pump to autosampler (0.17 × 200 mm) 0.005
Autosampler 1290 Infinity Autosampler with 20 µL loop 0.080 + injected volume
Capillary Autosampler to TCC (0.12 × 340 mm) 0.004
Capillary TCC to column (0.12 × 150 mm) 0.002
Thermostat Thermostat (3 µL) 0.003
Capillary Column to UV detector (0.12 × 280 mm) 0.003
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