Purification Solution - Developer's Guide 55
Calibration Procedures
6
Characterizing the delay volumes: Mixing point to UV detector and Column to UV detector
Preparative System
The Mixing point to UV detector tubing volume of the preparative system or
combined analytical and preparative 1260 system is calculated between the
T-connection that connects the preparative pumps and the UV detector cell.
The calculation of the Mixing point to UV detector tubing volume comprises
two steps (modify the tables in these steps for a customized setup):
1 Calculate Mixing point to column delay volume
UV Cell Max-Light Cartridge, 10 mm cell 0.001
Total 0.258 + injected volume
Tabl e 8 Preparative flow path of combined instrument:
Item Volume, mL
T-connection (preparative pumps) 0.002
T to mixer (0.6 × 40 mm) 0.011
Mixer (79835-87330) 0.75
Mixer to 2/10 valve (0.5 × 800 mm) 0.156
Valve to autosampler (0.5 × 600 mm) 0.117
Dual loop autosampler(G2258A), 5 mL loop 5.13
Autosampler to 2/10 valve (0.5 × 600 mm) 0.117
Valve to column (0.5 × 600 mm) 0.117
Total Preparative (mixing point to column) 6.40
Tabl e 9 Analytical flow path of combined instrument:
Item Volume, mL
Quat. Pump G1311B, Water/ACN gradient
1
p
0
/2760 + 0.742
Quat. pump to 2/10 valve (0.17 × 700) 0.016
Valve to autosampler (0.5 × 600 mm) 0.118
Part Description Volume, mL
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