56 Purification Solution - Developer's Guide
6
Calibration Procedures
Characterizing the delay volumes: Mixing point to UV detector and Column to UV detector
2 Calculate the final Mixing point to UV detector tubing volume as the sum
of:
• Mixing point to column tubing volume: step 1
• Column to UV detector tubing volume: see “Calculation of delay volume:
Column to UV detector” on page 50
Dual loop autosampler (G2258A), 50 µL loop 0.05
Autosampler to 2/10 valve (0.5 × 600 mm) 0.118
Valve to column (0.17 × 400 mm) 0.009
Total Analytical (mixing point to column)
p
0
/2760 + 1.053
1
For different pump use volumes from tables in Standard analytical system section.
Tabl e 10 UV/MSD-based instrument with UV detector in front of splitter (that is, in
preparative flow) or UV-based preparative instrument
Item Volume, mL
T-connection 0.002
Capillary to mixer (0.6 × 40 mm) 0.011
Mixer (79835-87330) 0.75
Capillary to autosampler (0.6 × 400 mm) 0.113
Dual loop utosampler(G2258A), 5 mL loop 5.13
Capillary to Column (0.5 × 800 mm) 0.156
Total Preparative (mixing point to column): 6.162
Tabl e 9 Analytical flow path of combined instrument:
Item Volume, mL
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