86 Purification Solution - Developer's Guide
7
Checkout Procedure
Acquire an analytical run using the Chemstation sequence
Set the recommended Stoptime and Flow as given above.
c Set the injection volume:
• Stand-alone analytical system: 1 μL injection (increase if necessary).
• Combined analytical and preparative system: 10 μL injection. If using
the dual-loop autosampler with a 10 μL injection loop, set Full loop with
overfill factor 5; otherwise, for larger loops set Partial loop filling to 10 μL
and set Plug Settings:
a Mark Plug Settings > Draw Plug before and after the sample.
b Set Plug Settings > Plug Volume: Enter the maximum plug volume:
Plug volume = ½[(Loop volume) – (Injection volume)]
That is, for a 10 μL injection to the 50 μL loop, set the plug volume
to 20 μL.
c Place plug solvent with 2 % acetonitrile (or Solvent A) into the
autosampler. Use another solvent if necessary.
d Enter the position of the plug vial in the autosampler method: Plug
Settings > Draw Plug from.
d Save the Method.
2 Dilute the Prep LC Standards #1 sample for analytical run with DMSO (or
other compatible solvent) based on the system and UV cell length:
• Combined analytical and preparative system (for 10 μL injection):
• 3 mm UV cell: dilute 10× (1 mL = 100 μL sample + 900 μL solvent).
• 10 mm UV cell: dilute 40× (1 mL = 25 μL sample + 975 μL solvent).
• Stand-alone analytical system (for 1 μL injection):
• 10 mm UV cell: dilute 20× (1 mL = 50 μL sample + 950 μL solvent).
Time (min) A (%) B (%)
0.00 98 2
0.17 98 2
1.83 2 98
2.20 2 98
2.30 98 2
Stoptime: 3.3 min
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