Purification Solution - Developer's Guide 87
Checkout Procedure
7
Acquire an analytical run using the Chemstation sequence
• 60 mm UV cell: dilute 100× (1 mL = 10 μL sample + 990 μL solvent).
3 Place 1 mL filtered sample in the autosampler.
4 Create a new sequence: Sequence > New Sequence Template
5 Set Sequence > Sequence Parameters (for example, folder path, shutdown
macro).
6 Complete the sequence table: Sequence > Sequence Table:
• Location: sample position.
• Sample name: Check-out sample
• Method name: select the analytical method (Analytical_Gradient)
• Inj/Location: 1
• Leave Sample amount field empty
• Click OK
7 Sequence > Save Sequence Template AsCheckout_Sample
8 Display the 263 nm UV profile (signal A) in the Online Plot.
9 Turn on the pump.
10 For the dual-loop autosampler:
a Equilibrate the upper loop:
a Switch the valve to the upper loop (right-click on the autosampler
diagram and select Switch Valve to Upper Loop; if the command is not
available, the upper loop is already selected).
b Apply a flow of 2mL/min for 5min
c Switch the valve to the lower loop
b Wash the needle (right-click on the autosampler diagram and select Wash
Needle > Flush Port > 15 s)
c Purge the needle (right-click on the autosampler diagram and select Start
Purging > 3×)
11 Set the target analytical flow (default is 1 mL/min for a 4.6 mm ID column
and 0.6 mL/min for a 2.1 mm ID column).
12 Equilibrate the column until the pressure and UV signal are stable.
13 RunControl > Run Sequence.
14 Rerun the sample at least once and check the results of the sequence
visually (peak shape, resolution). If the UV absorbance of five major peaks
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