Purification Solution - Developer's Guide 47
Calibration Procedures
6
Reviewing analytical results
3 Determine the column void volume, see “Characterizing the column
volume” on page 63
Determination of the column Porosity parameter for Purification software.
In the Purification software, the Porosity equals the column void volume
divided by the column geometric volume. A typical value for 4.6 mm and
21.2 mm ID ZORBAX StableBond (SB) C18 columns is 50 %.
4 Determine the delays for the analytical system and measure the fraction
collector delays for the preparative system, see “System Configuration and
Delay Time Calibration” on page 70
• Configure the purification systems in the Purification software.
• Determine the delay time for the Analytical systems.
• Calibrate the delay time for the Preparative systems.
5 Check-out sample test in Purification software, see “Checkout
Procedure” on page 83
a Acquire analytical run(s) in a ChemStation sequence.
b Set up and measure a focused gradient in Purification software for
separated analytical and preparative UV-only instrument.
c Set up and measure a focused gradient in Purification software for
combined and separated analytical and preparative MSD instruments.
Do not forget to remove the column from the flow path.
To Next Page
Loading...
Need help?
General
