78 Purification Solution - Developer's Guide
6
Calibration Procedures
System Configuration and Delay Time Calibration
5 For G1364B and G1364E: place an empty vial tray or well-plate tray in the
fraction collector.
FC Delay Calibration
1 From the Purification menu in the Chemstation, open the System Parameters
dialog box.
2 Select the preparative system configured in “Configuring the Preparative
System Parameters” on page 75.
3 In the Fraction collector calibration section, select the automated mode and
click Do calibration run.
4 In the Setup fraction collector delay calibration dialog box, enter the following:
• Location of calibration sample: the location of the Prep LC Standards #2
vial.
• Amount of calibration sample: an injection volume of 80 mL. Adjust if
necessary.
• Calibration base method: browse for the Prep_Purification method created
in “Preparing Default Purification Methods” on page 9.
• Set the Calibration run duration according to the flow and column. For a
21.2 × 50 mm column and 20 mL/min, use 1.5 min.
• If you are running the first calibration run, mark the Clean sampler and
equilibrate column before calibration check box. For subsequent calibration
runs, it is not necessary to repeat the cleaning.
5 Click Start calibration and then follow the instructions during the run.
6 When the calibration run is finished, click Evaluate.
7 In the Delay calibration data evaluation dialog box:
a Ensure that the peaks in the UV detector, fraction collector sensor
signals and, if present, the MSD and auxiliary signals are integrated and
identified (the integrated peak area is dark blue).
b MSD signal ion masses are predefined in the automatic evaluation
procedure for each calibration sample. If multiple ion masses are
expected, the procedure selects the most abundant one for the
evaluation:
• G1364B with Prep LC Standards #2 (5190-6887):
• Positive polarity: M+H (228.1).
• Negative polarity: M-H (226.1).
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