Purification Solution - Developer's Guide 95
Checkout Procedure
7
UV/MS Workflow: Focused gradient in Purification software
Minimal target candidate peak height in EIC default value was increased.
Change the value if necessary.
• Also check that the caffeine peak was identified in both the UV detector
and the MSD target ion species. If the UV and MSD are not well aligned,
then the problem can be in UV/MSD delay value or integration
parameters. If the UV signal is saturated, the integration parameters may
need to be changed.
15 Click Run again to submit the preparative task to the ChemStation run
queue.
16 When the preparative task is completed, check in Preparative Results that the
correct peak was focused and collected.
The table below lists the compounds in the Prep LC Standards #1 sample,
their most abundant isotopic masses, formulas and a typical ion species for
MS-based fraction collection:
Compound Most abundant
isotopic mass
Formula Typical Ion
species
Caffeine 194.1 C
8
H
10
N
4
O
2
M+H
Methyl 4-hydroxybenzoate 152.0 C
8
H
8
O
3
M-H
Ethyl 4-hydroxybenzoate 166.1 C
9
H
10
O
3
M-H
Propyl 4-hydroxybenzoate 180.1 C
10
H
12
O
3
M-H
Benzyl 4-hydroxybenzoate 228.1 C
14
H
12
O
3
M-H
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