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G1365B
Agilent Technologies G1365B User Manual
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Agile
nt T
e
ch
no
logies
20
06
Prin
ted in Germany
Editio
n 0
2
/2
00
6
*G1315-90006*
*G1315-90006*
G1
31
5-9
0
0
0
6
www
.agilent.com
Agilent T
e
chnologies
In This Book
This manual cont
ains
t
e
chnical ref
e
ren
c
e
inf
o
r
m
ation about t
h
e
A
g
ilen
t
1
200 Series diode
ar
ra
y det
e
ct
ors.
The manual describes t
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e
fo
l
l
o
w
i
n
g
:
•
int
r
o
c
d
u
cti
on and
specifications,
•
inst
allati
on,
•
usin
g an
d
opt
imi
zing
,
•
troubl
eshoot
ing
,
•
mai
n
ten
a
nce an
d
repai
r
,
•
parts i
d
enti
fi
cation
,
•
safety
an
d related
i
n
fo
rm
atio
n.
181
Table of Contents
Default Chapter
5
Table of Contents
5
1 Introduction to the Detector
11
Introduction to the Detector
12
Optical System Overview
13
Early Maintenance Feedback (EMF)
16
EMF Counters
16
Using the EMF Counters
16
Electrical Connections
18
Instrument Layout
20
2 Site Requirements and Specifications
21
Site Requirements
22
Physical Specifications
25
Performance Specifications
26
3 Installing the Detector
29
Unpacking the Detector
30
Damaged Packaging
30
Delivery Checklist
30
Optimizing the Stack Configuration
32
Installing the Detector
35
Flow Connections to the Detector
38
4 Using the Detector
43
Setting up an Analysis
44
Before Using the System
44
Requirements and Conditions
46
Optimization of the System
48
Preparing the HPLC System
49
Running the Sample and Verifying the Results
58
Special Settings of the Detector
59
Control Settings
59
Online Spectra
60
Analog Output Settings
61
Spectrum Settings
62
Peakwidth Settings
64
Slit Settings
65
Margin for Negative Absorbance Settings
66
Optimizing the Detector
66
5 How to Optimize the Detector
67
Optimizing the Detector Performance
68
Optimization Overview
69
Optimizing for Sensitivity, Selectivity, Linearity and Dispersion
71
Flow Cell Path Length
71
Peak Width (Response Time)
73
Sample and Reference Wavelength and Bandwidth
74
Slit Width
77
Optimizing Spectral Acquisition (DAD Only)
80
Margin for Negative Absorbance
81
Optimizing Selectivity
82
Quantifying Coeluting Peaks by Peak Suppression
82
Ratio Qualifiers for Selective Detection of Compound Classes
84
6 Troubleshooting and Diagnostics
87
Overview of the Detector's Indicators and Test Functions
88
Status Indicators
89
Power Supply Indicator
89
Detector Status Indicator
90
User Interfaces
91
Agilent LC Diagnostic Software
92
7 Maintenance and Repair
93
Introduction into Repairing the Detector
94
Simple Repairs
94
Exchanging Internal Parts
94
Warnings and Cautions
95
Cleaning the Detector
96
Using the ESD Strap
97
8 Maintenance
99
Overview of Maintenance
100
Exchanging a Lamp
101
Exchanging a Flow Cell
103
Maintenance of Standard, Semi-Micro or Micro Flow Cell
106
Maintenance of High Pressure Flow Cell
111
Replacing Capillaries on a Standard Flow Cell
114
Replacing Capillaries on a Semi-Micro, Micro and High Pressure Flow Cell
119
Nano Flow Cell - Replacing or Cleaning
123
Cleaning or Exchanging the Holmium Oxide Filter
128
Correcting Leaks
131
Replacing Leak Handling System Parts
132
Replacing the Interface Board
133
Replacing the Detector's Firmware
134
Tests & Calibrations
135
Intensity Test
136
Wavelength Verification and Recalibration
138
Holmium Oxide Test
139
Cell Test (Chemstation Only)
141
9 Parts and Materials for Maintenance
143
Overview of Maintenance Parts
144
Standard Flow Cell
146
Semi-Micro Flow Cell
148
Micro Flow Cell
150
High Pressure Flow Cell
152
Prep Flow Cell - SST
154
Prep Flow Cell - Quartz
156
Nano Flow Cells
158
Accessory Kit
162
A Appendix
166
General Safety Information
166
Lithium Batteries Information
169
The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC)
170
Radio Interference
171
Sound Emission
172
UV-Radiation
173
Solvent Information
174
Declaration of Conformity for HOX2 Filter
176
Agilent Technologies on Internet
177
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Agilent Technologies G1365B Specifications
General
Brand
Agilent Technologies
Model
G1365B
Category
Security Sensors
Language
English
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