1200 Series DAD and MWD User Manual 77
How to optimize the Detector 5
Slit Width
The detector has a variable slit at the entrance of the spectrograph. This is an
effective tool to adapt the detector to changing demand of different analytical
problems.
A narrow slit provides spectral resolution for analytes with very fine
structures in the absorbance spectrum. An example of such a spectrum is
benzene. The five main absorbance bands (fingers) are only 2.5 nm wide and
just 6 nm apart from each other.
Figure 30 Gradient Analysis of PTH-Amino Acids (1 pmol each), with and without Refer-
ence
1pmol each
Wavelength
267 nm
Reference 380 nm
Wavelength
267 nm
No reference
Time (min)
PTH-ASN
PTH-ARG
PTH-ALA
PTH-PRO
PTH-PHE
Grad.: 0.02 m KH
2
PO
4
/ACN from 12% ACN to 45% ACN in 12 min
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