1200 Series DAD and MWD User Manual 69
How to optimize the Detector 5
Optimization Overview
Tab le 11 Optimization Overview
Parameter Impact
1 Selection of flow cell
• Choose flow cell according to used column, see Figure 25.
• peak resolution versus sensitivity
2 Connection of flow cell
• For flow rates from 0.5 ml/min connect column using the
zero-dead-volume fittings of the detector.
• For small column i.d. (e.g 1 mm) the inlet capillary of the micro flow cell
can be connected directly to the column.
• chromatographic resolution
3 Setting the peak width (response time)
• Use peak width according Figure 25 as starting point.
• Set the peak-width close to the width of a narrow peak of interest in
your chromatogram.
• peak resolution versus sensitivity versus
disk space
4 Setting wavelength and bandwidth
• Sample wavelength:
• Never miss a peak by the use of a browser wavelength like 250 nm
with 100 nm bandwidth.
• Select specific wavelength with reduced bandwidth if you need
selectivity, e.g. 250,10 nm and 360,100 nm as reference wavelength.
• Set the sample wavelength to a peak or valley in the spectrum to get
best linearity for high concentrations.
• Reference wavelength:
• Select the reference wavelength with broad bandwidth (30...100 nm)
wavelength range where your analytes have little or no absorbance
(e.g. sample at 254 nm, reference at 320 nm).
• sensitivity versus selectivity
• sensitivity versus linearity
• baseline drift due to RI effects.
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