6 1200 Series DAD and MWD User Manual
Before Using the System 44
Requirements and Conditions 46
Optimization of the System 48
Preparing the HPLC System 49
Running the Sample and Verifying the Results 58
Special Settings of the Detector 59
Control Settings 59
Online Spectra 60
Analog Output Settings 61
Spectrum Settings 62
Peakwidth Settings 64
Slit Settings 65
Margin for Negative Absorbance Settings 66
Optimizing the Detector 66
5 How to optimize the Detector
Optimizing the Detector Performance 68
Optimization Overview 69
Optimizing for Sensitivity, Selectivity, Linearity and Dispersion 71
Flow Cell Path Length 71
Peak width (response time) 73
Sample and Reference Wavelength and Bandwidth 74
Slit Width 77
Optimizing Spectral Acquisition (DAD only) 80
Margin for Negative Absorbance 81
Optimizing Selectivity 82
Quantifying Coeluting Peaks by Peak Suppression 82
Ratio Qualifiers for Selective Detection of Compound
Classes
84
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