System Maintenance
112 NovoCyte Quanteon Flow Cytometer Operator's Guide
4 Power on the instrument and start NovoExpress software. After the
instrument is Ready, click Instrument > Fluidics Maintenance > Cleaning to
finish.
SIP cleaning apparatus directly contacts the biological samples which may
be biohazardous. Wear gloves when handling the SIP cleaning apparatus.
When cleaning the SIP cleaning apparatus, please be careful to prevent any
damages to the apparatus and sample injection probe.
Unclog the Flow Cell
When the instrument is in Ready status, click Instrument > Fluidics Maintenance >
Unclog from NovoExpress software, and the Unclog procedure will automatically
be executed. The progress of the procedure will be shown in the NovoExpress
status bar.
Figure 87. Unclog Function in NovoExpress
Sterilize Instrument Reagent Containers
It is very important to keep the instrument reagent free of contamination by
sterilizing the instrument reagent containers. This procedure is highly
recommended once a month or whenever there is a suspected bacteria growth or
possible contamination (e.g. by mishandling the containers). The reagent
containers need to be properly removed and sterilized using the following method
respectively.
Please refer to Instrument Start-Up in this guide for instruction on removing the
containers.
The procedures described below may vary if NovoCyte Fluidics Cart is used.
Please refer to NovoCyte Fluidics Cart Operator's Guide for more details.
1 Ensure the container is properly removed from the fluidics station. Wipe the
outer surface of the container using clean soft cloth soaked with 70% ethanol.
2 Empty the reagent container. Fill the NovoFlow container with at least 300 mL
of NovoClean solution (1X) and cap the bottle. Fill the NovoClean and
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