Introduction
NovoCyte Quanteon Flow Cytometer Operator's Guide 37
principle. Although the signals differ in intensity, the percentage of the FITC
spillover into the PE detector remains constant.
Figure 11. Spillover of Two FITC Signals of Different Intensities into PE Channels
The NovoExpress software provides flexible fluorescence compensation
functions. Users can modify the compensation matrix manually or use the
automatic compensation function. Fluorescence compensation can be conducted
during or after sample acquisition.
For more information on how to conduct fluorescence compensation, please refer
to Perform Fluorescence Compensation in this guide and NovoExpress Software
Guide for details.
Fluidics System
Agilent flow cytometer fluidics system consists of sample injection probe (SIP),
SIP cleaning apparatus, driving pumps, flow cell, solenoid valves, and a series of
tubings and connectors.
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