Introduction
38 NovoCyte Quanteon Flow Cytometer Operator's Guide
Sample Injection Probe (SIP) and SIP Cleaning Apparatus
The sample injection probe, which is a hollow stainless-steel tube, is used to
aspirate the sample from the test tube. When running samples, the sample
injection probe will move downward from the housing into the test tube, draw a
certain volume of the sample, and then retract back to the original position. By
default, the SIP cleaning apparatus (Figure 12) will automatically clean the inner
wall and the outer wall of the SIP after each sample acquisition. The waste fluid
generated by SIP cleaning procedure is collected to the waste container. This
automatic SIP cleaning function ensures minimal inter-sample carryover rate
(<0.1%) and eliminates tedious manual cleaning processes.
Figure 12. Sample Injection Probe (SIP) and SIP Cleaning Apparatus
To increase the sampling speed, user can turn off the default automatic rinsing
procedure that occurs after each sampling. To do so, uncheck the Rinse after
Sampling box in the Cytometer Control panel from the NovoExpress software.
The sample injection probe may perform multiple aspirations for one
sampling. Do not remove or change the sample tube until the entire
sampling process has completed.
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