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General| Brand | Agilent Technologies |
|---|---|
| Model | NovoCyte Quanteon |
| Category | Laboratory Equipment |
| Language | English |
This guide describes the information and procedures to install, operate, maintain, and troubleshoot NovoCyte Quanteon flow cytometer and associated standard accessories.
The following table lists the symbols which may be used in this guide.
This chapter provides overview of NovoCyte Quanteon flow cytometer, including basic instrument operation principle, system requirements, and system components.
The optics system consists of lasers, optical lenses, beam shaping optics, light collection optics, optical filters, and photodetectors.
Explains spectral overlap and the need for fluorescence compensation.
Describes the components of the fluidics system.
Describes the electronics system and signal processing.
Details software, workstation, and fluidics station requirements.
Lists the main system components including the instrument, fluidics station, and workstation.
Lists critical warnings and precautions for instrument installation.
Specifies the minimum space needed for installation.
Outlines the recommended operating and storage environmental conditions.
Provides guidance on safely moving and handling the instrument.
Details how to connect the workstation to the instrument and monitor.
Explains how to connect the fluidics station to the instrument.
Details how to connect the instrument to the power supply.
Guides through the initial steps to power on and prepare the instrument.
Provides instructions for refilling reagent containers.
Guides on how to empty the waste container.
Explains how to check and customize the instrument's optical configuration.
Explains the purpose and procedure for instrument quality control.
Guides on how to properly shut down the instrument.
Describes the example test using peripheral blood lymphocytes.
Guides on creating a new experiment file in NovoExpress software.
Details how to set detection parameters and channel aliases.
Explains how to set stop conditions, flow rate, and thresholds.
Guides on how to initiate sample acquisition.
Explains automatic and manual methods for fluorescence compensation.
Introduces data analysis tools and plots in NovoExpress software.
Details manual and automatic methods for exporting data files.
Lists routine maintenance tasks and their recommended frequency.
Outlines monthly preventative maintenance procedures for optimal system condition.
Guides on replacing fluidic system consumables when prompted.
Lists maintenance tasks performed as needed based on specific conditions.
Explains the debubble procedure for removing air bubbles.
Details the decontamination procedure for the fluidics system.
Explains how to recalibrate the fluidics station.
Procedure to clean the flow cell or sampling tubing.
Addresses issues when the instrument QC test fails.
Troubleshoots scenarios with few or no detected events.
Troubleshoots excessive debris appearing in the data display.
Addresses issues when NovoExpress shows instrument as not connected.
Troubleshoots leakage issues originating from the SIP.
Lists the version history of the manual.
Details the laser wavelengths and detectable fluorochromes.
Lists optical filters and recommended fluorochromes for each detector.
Lists optics, laser, and fluidics specifications.
Lists specifications for the workstation and monitor.
Lists specifications for the fluidics station.
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