Troubleshooting
134 NovoCyte Quanteon Flow Cytometer Operator's Guide
NovoFlow container is empty
Refill the NovoFlow container following the
procedures described in associated instrument
operator's guide.
NovoFlow container is not
correctly connected to the
instrument and the NovoFlow
solution does not flow through
the tubing
Reconnect the NovoFlow quick coupling
connectors to ensure the NovoFlow container is
correctly connected to the instrument.
Execute Prime function from the NovoExpress
Software.
Sample Injection Probe is
clogged
Run NovoClean as sample to remove the things
clogged in SIP.
Execute the Unclog function from the
NovoExpress Software.
Clean the leaked fluid with wipes and properly
reconnect the sample tubing.
Air bubbles exist in the
Sampling Pump
Execute Debubble function from NovoExpress
Software.
Air bubbles exist in the flow
cell
Execute the Debubble function from
NovoExpress Software.
Execute the Unclog function from NovoExpress
software
Improper sample preparation
Re-prepare the sample properly.
The sample flow rate is set
too high
Lower the sample flow rate setting in the
NovoExpress software.
Excessive debris in
display
Sheath in-line filter is dirty
Replace the sheath in-line filter following the
procedures described in associated instrument
operator's guide.
The threshold is set too low
Increase the threshold setting and re-collect the
sample.
Fluidics system tubing is dirty
• Execute Rinse function from NovoExpress
Software.
• Execute Cleaning function from
NovoExpress Software.
• Execute Decontamination function from
NovoExpress Software.
The threshold is set too low
Increase the threshold setting and re-collect the
sample.
Sample concentration is too
high
Re-prepare the sample to appropriate
concentration or dilute the sample.
Sample flow rate is too high
Reduce the sample flow rate.
NovoExpress
displays Instrument
Workstation is in hibernation
causing the interruption of
• Restart NovoExpress Software.
• Restart workstation.
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