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7500 Fast
Applied Biosystems 7500 Fast Getting Started
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Applied
Biosystems
7500/7500 Fast
Real-T
ime PCR System
Standar
d Curve Experiments
Get Started
Design the
Standard Curve
Experiment
Prepar
e the
Reactions
Run the
Experiment
Analyze the
Experiment
Getting Started Guide
2
4
Table of Contents
Default Chapter
5
Table of Contents
5
How to Use this Guide
9
Preface
9
How to Obtain more Information
11
How to Obtain Support
13
Safety Conventions Used in this Document
14
Symbols on Instruments
15
Safety Labels on Instruments
16
General Instrument Safety
18
Chemical Safety
19
Chemical Waste Safety
21
Electrical Safety
22
Physical Hazard Safety
22
Biological Hazard Safety
23
Workstation Safety
23
Safety and Electromagnetic Compatibility (EMC) Standards
24
Chapter 1 Get Started
25
Get Started
25
About the 7500/7500 Fast System
26
Supported Consumables
28
About Standard Curve Experiments
31
How to Use this Guide
35
About the Example Standard Curve Experiment
36
Example Experiment Workflow
38
Design the Standard Curve Experiment
41
Chapter 2 Design the Standard Curve Experiment
42
Chapter Overview
42
Create a New Experiment
43
Define the Experiment Properties
44
Define the Methods and Materials
46
Set up the Targets
48
Set up the Standards
50
Set up the Samples
52
Set up the Run Method
54
Review the Reaction Setup
56
Order Materials for the Experiment
61
Finish the Design Wizard
64
Chapter 3 Prepare the Reactions
67
Chapter Overview
68
Prepare the Sample Dilutions
69
Prepare the Standard Dilution Series
71
Prepare the Reaction MIX
73
Prepare the Reaction Plate
75
Chapter 4 Run the Experiment
79
Chapter Overview
80
Prepare for the Run
81
Enable the Notification Settings (Optional)
83
Monitor the Run
85
Start the Run
85
Unload the Instrument
88
Chapter 5 Analyze the Experiment
89
Chapter Overview
90
Section 5.1: Review Results
91
Section 5.1 Review Results
91
Analyze the Experiment
92
View the Standard Curve
95
View the Amplification Plot
98
View the Well Table
105
Publish the Data
107
Section 5.2: Troubleshoot (if Needed)
109
Section 5.2 Troubleshoot (if Needed)
109
View the Analysis Settings
110
View the QC Summary
112
Omit Wells from the Analysis
114
View the Multicomponent Plot
116
View the Raw Data Plot
118
Appendix A Alternate Experiment Workflows
121
Advanced Setup Workflow
122
Quickstart Workflow
124
Template Workflow
126
Export/Import Workflow
128
Bibliography
131
Glossary
133
Index
147
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Applied Biosystems 7500 Fast Specifications
General
Brand
Applied Biosystems
Model
7500 Fast
Category
Laboratory Equipment
Language
English
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