Chapter 5 Analyze the Experiment
Publish the Data
83
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
Notes
Analysis
Guidelines
When you analyze your own standard curve experiment, group the wells by:
• Replicate – The software groups the wells by replicate: negative controls,
standards, and samples. Look in the Quantity columns to make sure the quantity
values for each replicate group are similar. This indicates tight precision.
• Flag – The software groups the flagged and unflagged wells. A flag indicates that
the software found an error in the flagged well. For a description of the 7500
software flags, see “View the QC Summary” on page 88.
•
C
T
– The threshold cycle (C
T
) is the PCR cycle number at which the fluorescence
level meets the threshold. A C
T
value >8 and <35 is desirable. A C
T
value <8 indicates
that there is too much template in the reaction. A C
T
value >35 indicates a low amount
of target in the reaction; for C
T
values >35, expect a higher standard deviation.
For More
Information
For more information on the Well Table, open the 7500 Software Help by clicking or
pressing F1.
Publish the Data
You can publish the experiment data in several ways:
• Save the plot as an image file
• Print the plot
• Print the plate layout
• Create slides
• Print a report
• Export data
For information on performing these procedures, open the 7500 Software Help by
clicking or pressing F1.
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