Applied Biosystems 7500 Fast Getting Started

Chapter 4 Run the Experiment
Start the Run
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Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
Notes
Start the Run
IMPORTANT! While the 7500/7500 Fast instrument is performing a run, do not create
experiments, perform maintenance, or allow the computer to run antivirus software or to
enter hibernation mode. Performing such activities while the instrument is running an
experiment will cause gaps in data collection.
To start your 7500/7500 Fast instrument:
1. In the 7500 software, click in the navigation pane.
2. Click START RUN .
Monitor the Run
You can view the progress of the run in real time as described below. During the run,
periodically view all three available plots from the 7500 software for potential problems.
1
2
To… A ct i on
Stop the run 1. In the 7500 software, click STOP RUN.
2. In the Stop Run dialog box, click one of the following:
– Stop Immediately to stop the run immediately.
– Stop after Current Cycle/Hold to stop the run after the
current cycle or hold.
– Cancel to continue the run.
View amplification data
in real time
Select Amplification Plot.
See “About the Amplification Plot Screen” on page 62.
View progress of the
run in the Run Method
screen
Select Run Method.
See “About the Run Method Screen” on page 63.

Applied Biosystems 7500 Fast Specifications

General

Brand	Applied Biosystems
Model	7500 Fast
Category	Laboratory Equipment
Language	English