Applied Biosystems 7500 Fast Getting Started

Chapter 5 Analyze the Experiment
Chapter Overview
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
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Notes
Chapter Overview
The 7500 software analyzes the data using the standard curve quantitation method.
Section 1 of this chapter explains how to review the analyzed data using several of the
analysis screens and how to publish the data. If you obtain questionable results, Section 2
of this chapter explains how to perform some troubleshooting steps.
Example
Experiment
Workflow
Design the Experiment (Chapter 2)
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Start Experiment
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Prepare the Reactions (Chapter 3)
Run the Experiment (Chapter 4)
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End Experiment
Analyze the Experiment (Chapter 5)
Section 1, Review Results:
1. Analyze.
2. View the standard curve.
3. View the amplification plot.
4. View the results in a table.
5. Publish the data.
Section 2, Troubleshoot (If Needed):
1. View the analysis settings; adjust the baseline/threshold.
2. View the quality summary.
3. Omit wells.
4. View the multicomponent plot.
5. View the raw data plot.

Applied Biosystems 7500 Fast Specifications

General

Brand	Applied Biosystems
Model	7500 Fast
Category	Laboratory Equipment
Language	English