Applied Biosystems 7500 Fast Getting Started

Chapter 1 Get Started
How to Use This Guide
11
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
Notes
How to Use This Guide
This guide functions as both a tutorial and as a guide for performing your own
experiments.
Using This Guide
as a Tutorial
By using the example experiment data provided with the 7500 software, you can use this
guide as a tutorial for performing a standard curve experiment on a 7500/7500 Fast
system. Follow the procedures in Chapters 2 through 5:
For more information, see “About the Example Standard Curve Experiment” on page 12.
Using This Guide
with Your Own
Experiments
After completing the tutorial exercises in Chapters 2 through 5, you can use this guide to
lead you through your own standard curve experiments. Each procedure in Chapters 2
through 5 includes a set of guidelines that you can use to perform your own experiments.
Additionally, you can use one of the other workflows provided in the 7500 software to
perform your experiments. The table below provides a summary of all the workflows
available in the 7500 software.
Chapter Procedure
2 Design the experiment using the Design Wizard in the 7500 software.
3 Prepare the experiment, using the reagents and volumes calculated by the
Design Wizard in Chapter 2.
4 Run the experiment on a 7500/7500 Fast instrument.
5 Analyze the results.
Workflow Description See...
Design Wizard Set up a new experiment with guidance from the software.
The Design Wizard guides you through best practices as
you create your own experiment. The Design Wizard is
recommended for new users.
Note: Design options are more limited in the Design
Wizard than in Advanced Setup.
Chapter 2
Advanced
Setup
Set up a new experiment using advanced options.
Advanced Setup allows design flexibility as you create
your own experiment. Advanced Setup is recommended
for experienced users.
page 98
QuickStart Run a new experiment with no plate setup information. If
desired, you can add all design parameters after the run.
page 100
Template Set up a new experiment using setup information from a
template.
page 102
Export/Import Import experiment designs from ASCII text files that
contain experiment setup information.
page 104

Applied Biosystems 7500 Fast Specifications

General

Brand	Applied Biosystems
Model	7500 Fast
Category	Laboratory Equipment
Language	English