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Applied Biosystems 7500 Fast Getting Started

Applied Biosystems 7500 Fast
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Chapter 5
65
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
Notes
Analyze the Experiment
This chapter covers:
Chapter Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Section 5.1 Review Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Analyze the Experiment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
View the Standard Curve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
View the Amplification Plot . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
View the Well Table . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Publish the Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Section 5.2 Troubleshoot (If Needed) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
View the Analysis Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
View the QC Summary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
Omit Wells from the Analysis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
View the Multicomponent Plot . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
View the Raw Data Plot . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
Note: For more information about any of the topics discussed in this guide, open the
Help from within Applied Biosystems 7500/7500 Fast Real-Time PCR Software v2.0 by
pressing F1, clicking in the toolbar, or selecting Help7500 Software Help.

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Applied Biosystems 7500 Fast Specifications

General IconGeneral
BrandApplied Biosystems
Model7500 Fast
CategoryLaboratory Equipment
LanguageEnglish

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