Chapter 3 Prepare the Reactions
Chapter Overview
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
44
Notes
Chapter Overview
This chapter explains how to prepare the PCR reactions for the standard curve example
experiment and provides guidelines for how to prepare the PCR reactions for your own
standard curve experiment.
Example
Experiment
Workflow
Design the Experiment (Chapter 2)
Start Experiment
Run the Experiment (Chapter 4)
Analyze the Experiment (Chapter 5)
End Experiment
Prepare the Reactions (Chapter 3)
1. Prepare the sample dilutions.
2. Prepare the standard dilution series.
3. Prepare the reaction mix for each target assay.
4. Prepare the reaction plate.