Chapter 1 Get Started
Example Experiment Workflow
15
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
Notes
End Experiment
Analyze the Experiment (Chapter 5)
Section 1, Review Results:
1. Analyze.
2. View the standard curve.
3. View the amplification plot.
4. View the results in a table.
5. Publish the data.
Section 2, Review Multiple Experiments Results:
1. Create a study.
2. Define replicates.
3. Analyze.
4. View the gene expression data.
5. View the experiment data.
6. View the multicomponent data.
7. View multiple plots.
8. View the quality summary.
9. Publish the data.
Section 3, Troubleshoot (If Needed):
1. View the analysis settings; adjust the baseline/threshold.
2. View the quality summary.
3. Omit wells.
4. View the multicomponent plot.
5. View the raw data plot.
Run the Experiment (Chapter 4)
1. Prepare for the run.
2. Enable the notification settings (Optional).
3. Start the run.
4. Monitor the run.
5. Unload the instrument and transfer the data.
(see page 14)