Applied Biosystems 7500 Fast Getting Started

Chapter 3 Prepare the Reactions
Prepare the Reaction Plate
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
54
Notes
• If you use optical adhesive film, seal each reaction plate as follows:
a. Place the reaction plate onto the center of the 96-well base.
b. Load the reaction plate as desired.
c. Remove a single optical adhesive film (film) from the box.
Fold back one of the end-tabs. Hold the film with its backing
side up.
d. In one continuous movement, peel back the white
protective backing from the center sealing surface. Do not
touch the center sealing surface.
IMPORTANT! Improper peeling of the optical adhesive film may result in
haziness, but does not affect results. Haziness disappears when the film comes
into contact with the heated cover in the instrument.
e. While holding the film by the end-tabs, lower the film onto
the reaction plate (adhesive side facing the reaction plate).
Be sure the film completely covers all wells of the reaction
plate.
f. While applying firm pressure, move the applicator slowly
across the film, horizontally and vertically, to ensure good
contact between the film and the entire surface of the
reaction plate.
g. While using the applicator to hold the edge of the film in
place, grasp one end of the end-tab and pull up and away
sharply. Repeat for the other end-tab.
h. Repeat step f to ensure a tight, evaporation-free seal. While
applying firm pressure, run the edge of the applicator
along all four sides of the outside border of the film.
Note: Optical adhesive films do not adhere on contact. The films require the
application of pressure to ensure a tight seal.
i. Inspect the reaction plate to be sure all wells are sealed. You should see an
imprint of all wells on the surface of the film.
For More
Information
For more information on:
• Preparing the reaction plate – Refer to the protocol appropriate for the reagents you
are using in the PCR reactions:
–TaqMan
®
Gene Expression Assays Protocol
– Custom TaqMan
®
Gene Expression Assays Protocol
• Consumables – See “Supported Consumables” on page 4.
• Using Advanced Setup to change the plate layout – See page 98.

Applied Biosystems 7500 Fast Specifications

General

Brand	Applied Biosystems
Model	7500 Fast
Category	Laboratory Equipment
Language	English