Applied Biosystems 7500 - Page 111

Chapter 5 Analyze the Experiment
View the Analysis Settings
87
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
Notes
Analysis
Guidelines
Unless you have already determined the optimal settings for your experiment, use the
default analysis settings in the 7500 software. If the default settings are not suitable for
your experiment, you can change the settings. Use the:
• C
T
Settings tab to manually set the threshold and baseline. When manually setting the
threshold and baseline, Applied Biosystems recommends the following:
• Flag Settings tab to:
– Adjust the sensitivity so that more wells or fewer wells are flagged.
– Change the flags that are applied by the 7500 software.
• Advanced Settings tab to change baseline settings well by well.
For More
Information
For more information on the analysis settings, open the 7500 Software Help by pressing
F1 when the Analysis Settings dialog box is open.
Setting Recommendation
Threshold Enter a value for the threshold so that the threshold is:
• Above the background.
• Below the plateau and linear regions of the amplification curve.
• Within the exponential phase of the amplification curve.
Baseline Select the Start Cycle and End Cycle values so that the baseline
ends before significant fluorescence is detected.